With deep pleasure and gratitude I would like to thank Mr. Kunal Dixit, Director, IBI BIosolutions, Chandigarh. Extreme dedication of Mr. Deepak Sethi for his work constantly influenced me to work hard at IBI Biosolutions.

It is a great pleasure for me to acknowledge my heartiest thanks to Prof. Pradeep K. Sharma, H.o.D., Department of Microbiology, C. C. S. University, Meerut and I am very thankful to Prof. Amar P. Garg, for guiding and helping me all through my course work, for providing me an opportunity to work in such an esteemed Bioinformatics company and to avail the facilities at IBI Biosolutions, Chandigarh and for their kindness and providing necessary facilities to undertake this study.

I am heartly thankful to Prof. Henery Paulus, Bostan Biomedical Research Institute, and Prof. Shmuel Pietrokovskifor, The Weizmann Institute of Science, Rehovot Israel for his guidance and supports, which very much helps me to work on this project.

I am extremely thankful to Mr. Rajeev Gautam, Mr. Ajay Shukla, Ms. Anuska Das Gupta and Ms. Ila for his guidance, cooperation, incessant encouragement in clarifying the doubts and kind assistance in the form of knowledge in the subject. I thank him for his constant support given for the completion of this thesis.

My heartiest thanks goes to my senior Mr. Nitin Bhardwaj, and my collogues at IBI Biosolutions Mr. Chirag Choudhary, Mr. Ashok Yadav, Mr. Abhishek Verma, Ms. Supriya, Ms. Kshama, and Ms. Gurpreet for their cheerful companionship and making my stay at IBI Biosolutions a memorable experience and my collogues at Department of Microbiology Mr. Sumit Malik, Ms. Preeti Rana, Mr. Ajay Kumar and Mr. Sachin Kumar.

I specially thanks to Mr.Anuj Garg, Department of Microbiology, C.C.S. University, Meerut for his valuable guidence, inspiration and support. His motivations are unforgetable for me.

At last but not least my special thanks to my family and all my friends friends who taught me to work hard and supported me during my work.